Dr jing yu pan Activator#

The enzymatic products of three Leloir genes act sequentially to convert galactose to glucose-6-phosphate, which enters glycolysis. In the presence of galactose, Gal3 relieves this repression of Gal4 ( Sil et al., 1999 Peng and Hopper, 2000 Sellick and Reece, 2006), thereby activating the transcription of target GAL genes such as GAL1, GAL7, and GAL10 ( Johnston, 1987). In the absence of galactose, Gal80 prevents transcriptional activation of the pathway by means of a physical interaction with Gal4 ( Platt and Reece, 1998). This pathway is controlled through the Gal4 activator, a member of the Cys6 zinc finger class of transcription factors found only in fungi ( Burger et al., 1991), as well as a sensor (Gal3) and a repressor (Gal80). The GAL pathway of the budding yeast Saccharomyces cerevisiae is a favorite textbook example of the regulation of gene expression in eukaryotes.

Galactose is a monosaccharide that is abundant in nature and is found in many forms. albicans evolves transcriptional programs to colonize diverse host niches. Rep1-Cga1 mode of regulation represents a new example of network rewiring in fungi, which provides insight into how C.

Instead, it provides a scaffold to recruit different factors for transcriptional regulation. Rep1 itself does not possess transcriptional activity. Rep1 and Cga1 are conserved across the CTG species.

Dr jing yu pan Activator#

The net result of this interaction is the recruitment of a transcriptional activator Cga1 (Candida galactose gene activator, orf19.4959) and transcription of the GAL genes proceeds. Rep1 recognizes galactose via a direct physical interaction. It is presented at the GAL gene promoters independent of the presence of galactose. Here, we find that Rep1, a Ndt80-like family transcription factor, serves as a galactose sensor in the commensal-pathogenic fungus Candida albicans. While Gal4 mode has been well-characterized in Saccharomycetaceae clade, little is known about the regulation of the GAL pathway in other yeasts. The GAL pathway of yeasts has long served as a model system for understanding of how regulatory mode of eukaryotic metabolic pathways evolves.