Single-cell suspensions or thorough homogenates are obtained using single-use, sterile C Tubes or M Tubes.

8 samples were sequenced at 10x Genomics and included on these runs.

The HTML run reports from Cell Ranger have been uploaded to the project, under the Analyses tab. All eight positions can be operated independently. Access the 10x Genomics Cell Ranger html report for the Chromium Single Cell Gene Expression dataset. It also allows for the creation of user-defined programs. The Miltenyi Biotec GentleMACS™ Octo Dissociator with Heaters is a benchtop instrument for the fully automated and standardized tissue dissociation or homogenization of up to eight samples.Įquipped with eight individual heating units and ready-to-use gentleMACS programs, it offers a fully-automated workflow for tissue dissociation. Miltenyi Biotec GentleMACS Octo Tissue Dissociator By appointment, researchers may utilize our GentleMACS instrument to develop fully automated protocols for dissociating their tissues of interest into cell suspensions ready for downstream applications. For further details and pricing, please contact us.Īs part of our mission to provide high quality single cell sequencing data, we offer walk-up access to our Miltenyi Biotech GentleMACS Octo tissue dissociator with heaters. We offer detailed analysis of your single cell data. We collaborate with the Botson University Microarray & Sequencing Core to provide you with the best sequencing for your library via Illumina NextSeq 5 platforms. 10X Chromium Single Cell solutions can capture between 500 – 10,000 cells from samples with cell concentrations of 100 – 2000 cells/uL. This enables novel discovery of gene expression dynamics and molecular profiling of individual cell types in an affordable and simple workflow. Specifically, The Chromium Single Cell 3’ Solution provides a comprehensive, scalable solution via high-throughput single cell characterization and gene expression profiling. Single Cell Services 10X Genomics Chromium™ Systemġ0x Genomics’ Chromium System is a unique system that partitions cells and prepares sequencing libraries in parallel such that all fragments produced within a partition share a common barcode. Uncover critical insight with our services. We offer multiple single cell library preparation options and next-generation Illumina sequencing via our collaboration with the BU Microarray & Sequencing Core. As a high-throughput core, we provide an array of services to get you from sample to high-quality single cell gene expression data and/or analysis.

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